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The Assessment of Pain in
Mice using Telemetry
M. Arras
Institute of Laboratory Animal Science
University of Zurich
…temporarily (2 weeks) paralyzed hind
legs caused by activated transgene
Institute of Laboratory Animal Science
Pain?
University of Zurich
Pain?
Institute of Laboratory Animal Science
Answers
no pain
12
intermediate
pain
severe pain
16
pain therapy
9
sacrifice
4
11
University of Zurich
Goal of the study
Objective measurements of pain and
suffering in laboratory mice
Institute of Laboratory Animal Science
University of Zurich
Telemetry
measurements:
30 sec/5 min
Institute of Laboratory Animal Science
University of Zurich
Model
transmitter
implantation
intermediate
pain
recovery
no pain
Institute of Laboratory Animal Science
vasectomy
mild
pain
University of Zurich
Parameters
• What we measure
– heart rate (ECG)
– body temperature
– activity
• How we interpret
– circadian rhythm
Institute of Laboratory Animal Science
University of Zurich
900
Heart
Rate
800
700
600
600
500
500
400
300
1day
200
heart rate [bpm]
700
300
200
1da
y
6 days after transmitter implantation
900
900
chronic tumour disease
800
700
700
600
600
500
500
400
300
intermediate pain
400
6d, control
800
heart rate [bpm]
normal
1day
200
heart rate [bpm]
heart rate [bpm]
800
900
6d tumor disease
Institute of Laboratory Animal Science
mild pain
400
300
1day
200
6d, vasectomy
University of Zurich
39
39.0
intermediate pain
38.0
37
37.0
36
35
1day
34
body temperature [°C]
body temperature [°C]
normal
38
36.0
35.0
34.0
6d, control
Body
Temp.
39
39
mild pain
38
38
37
37
36
35
1da
y
6 d, tumor disease
body temperature [°C]
body temperature [°C]
chronic tumour disease
34
1da
y
6 d after transmitter
implantation
Institute of Laboratory Animal Science
36
35
34
1da
6d, after y
vasectomy
University of Zurich
12
10
12
normal
8
1day
6
6
4
4
2
2
activity
activity
8
10
0
6 d after transmitter implantation
12
12
chronic tumour disease
mild pain
10
10
1da
y
8
6
6
4
4
2
2
activity
activity
8
1da
y
0
6d, normal
Activity
intermediate pain
0
6d, tumor disease
Institute of Laboratory Animal Science
1da
y
0
6d, after vasectomy
University of Zurich
Indicators of pain
PHYSIOLOGY
heart rate
body temperature
BEHAVIOR
activity
behavioral tests
score sheet
Institute of Laboratory Animal Science
GENE REGULATION
identification of genes
related to pain and
stress in transgenic mice
University of Zurich
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