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Types of Light Microscopy Jenna Moranski Compound Light Microscope • What can be viewed • Transparent samples • Dead specimen • Must be small enough to fit on a slide • Can zoom in up to 1000X Bright Field Microscope • The specimen is dark and the light is bright around it • Staining the specimen is normally required • Can be used to view: • Fixed specimen • Live samples Dark Field Microscope • Light is directed at an angle toward the sample • The condenser lens transmits only the light reflected off the sample • Can be used to view • Yeast • Bacteria • Pond water Phase Contrast Microscope • Most living organisms are more easily seen in a phase contrast microscope • Used to view • Cilia • Flagella • Amoebae Differential Interface Contrast Microscope • Helps to make contrast in transparent, unstained specimen • Used to view • Living or stained specimens that are hard to clearly see in bright field microscopes Fluorescence Microscope • Uses a much higher intensity light source than light microscopes • The light excites the fluorescent parts in the specimen • Can be used to view • Genetic material in a cell • Structural components of cells Confocal Microscope • Makes it possible to see inside living or dead cells • Blocks out-of-focus light from inside the cell
