* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Datasheet - LifeSensors
Silencer (genetics) wikipedia , lookup
Gene regulatory network wikipedia , lookup
Gene expression wikipedia , lookup
Biochemistry wikipedia , lookup
Protein adsorption wikipedia , lookup
Peptide synthesis wikipedia , lookup
Expression vector wikipedia , lookup
Intrinsically disordered proteins wikipedia , lookup
Amino acid synthesis wikipedia , lookup
Protein moonlighting wikipedia , lookup
Two-hybrid screening wikipedia , lookup
Metalloprotein wikipedia , lookup
Western blot wikipedia , lookup
Cell-penetrating peptide wikipedia , lookup
Protein–protein interaction wikipedia , lookup
Ribosomally synthesized and post-translationally modified peptides wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
List of types of proteins wikipedia , lookup
NEDD8-Vinyl Methyl Ester, human recombinant Cat. # SI640 Background: Application: Interferon stimulated gene 15 (ISG15) is a member of the ubiquitin-like protein family whose expression is increased following stimulation with type 1 Interferons. ISG15-VME is synthesized by the conjugation of 4-amino-but-2-enoic acid methyl ester to the C-terminus of ISG15G156. Binding of ISG15-VME by deISGenylases positions the reactive vinyl bond next to the sulfhydryl-group of the active site cysteine. Nucleophilic attack by the sulfhydryl on the vinyl bond produces a stable, covalent thioether linkage between ISG15 and the deISGenylase. Thus ISG15-VME is a potent suicide inhibitor of deISGenylases. This inhibitor is useful for labeling deISGenylases in situ as well as preserving the ISGenylated proteins for analysis or purification. Product Information Purity: > 95% by HPLC Molecular Weight: 9100 Da by MS Physical State: Lyophilized powder Quantity: 50 g Solubility: > 1 mg/mL in aqueous buffer Storage: Dry, -20 C. Solution, -80 C. Avoid repeated freeze/thaw cycles o o References 1) Borodovsky A., et al. Small-molecule inhibitors and probes for ubiquitin- and ubiquitin-like-specific proteases. Chem. Biochem. 2005 6: p287291 2) Hemelaar J., et al. Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins. Mol. Cell. Bio. 2004 24: p84-95 3) Gan-Erdene T., et al. Identification and characterization of DEN1, a deneddylase of the ULP family. J. Biol. Chem. 2003 278: p28892-28900 4) Mendoza H.M., et al. NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins. J. Biol. Chem. 2003 278: p25637-25643 5) Wang G., et al. Solid-phase synthesis of peptide vinyl sulfones as potential inhibitors and activity-based probes of cysteine proteases. Org. Lett. 2003 5: p737-740 6) Wu K., et al. DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1. J. Biol. Chem. 2003 278: p28882-288891 7) Borodovsky A., et al. Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002 9: p1149-1159 All products are for research use only not intended for human or animal diagnostic or therapeutic uses Copyright © 2009 LifeSensors, Inc. All Rights Reserved