Download Examensarbete Activity of carbonic anhydrase II in presence of

Examensarbete
Activity of carbonic anhydrase II in presence of nanoparticles. Coating effects
The interaction of condensed materials with biological systems has long been a subject of
scientific and practical interest. The increase development and exposure of drug delivery
systems requires a thoughtful approximation to the possible consequences. Even though
those aggregates have a specific function, drug delivery, biocatalysis, etc.; unexpected
effects can rise from the exposure of those systems to living organism and in particular to
the performance of highly functional molecules as enzymes.
An important feature of nanomaterials is the protective coating. Many nanoparticles need
an extra organic layer in order to gain colloidal stability in solution and avoid coalescence
and precipitation. Therefore, for the same functional core material a series of coatings can
be found depending on the application. The primary interaction of surface active molecules
such as enzymes will consequently be the stabilizing layer. As a consequence of this, the
kinetic effect upon adsorption must be related to the physicochemical characteristics of this
coating.
The first step in this project is to analyze
the effect of the interaction between
colloidal structures such as metallic
nanoparticles and enzymes. The activity
and performance of enzymes can be
extremely sensitive to small changes in
protein conformation. The association to
surfaces can cause such modifications and
severely modify the protein functioning.
Using
time-resolved
spectroscopic
methods you will investigate how kinetics
effects correlate with changes in
conformation upon binding.
HCA II
Au@PSS
NPs
The association process between carbonic anhydrase II and gold polyelectrolyte coated
nanoparticles will be studied as well as the changes in enzyme conformation. These changes
will be correlated to the kinetics effects observed.
The same enzymatic reaction will be analyzed in a system mimicking a biological fluid. A
neutral (non- reacting) protein would be introduced in order to study the effect of the
competition between the proteins in solution for the particle surface. The final goal is to
relate the on and off rates and the binding constants with the overall kinetic picture.
Techniques.
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UV-spectroscopy
CD spectroscopy
Dynamic light scattering
Isothermal calorimetry
Surface Plasmon resonance
For further information, please contact me at [email protected]