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KAPA PURE BEADS
Attract what matters.
Benefits include:
KAPA Pure Beads offer a tunable and highly
consistent solution for reaction purification and
size selection in DNA and RNA next-generation
sequencing library construction workflows.
• high recovery of single- and double-stranded DNA
(1 ng – 5 µg) in a single cleanup
• fast and efficient cleanups to remove unwanted
reaction components
• easy substitution into bead-based workflows
• enables adjustable size selection
• automation friendly
Seamless Integration into NGS Workflows
• Compatible with all KAPA DNA and RNA library preparation protocols
• Achieve equivalent yields and size distribution in comparison to Agencourt® AMPure® XP
• Readily incorporated into existing automation applications
A50 50
B
40 40
Post-ligation Yield (nM)
Post-ligation Yield (nM)
Post-ligation Yield (nM)
Post-ligation Yield (nM)
60 60
20 20
40 40
10 10
20 20
0 0
0 0
KAPA
KAPA
Pure
Pure
Beads
Beads
160160
140140
KAPA Pure Beads provides equivalent
performance to Agencourt® AMPure® XP
in both DNA and RNA workflows.
80 80
30 30
180180
100100
KAPA
KAPA
PurePure
KAPA
KAPA
PurePure
KAPA
KAPA
PurePure
AMPure
AMPure
XP XP
KAPA
KAPA
Pure
Pure
Beads
Beads
180180
AMPure
AMPure
XP XP
AMPure
AMPure
XP XP
AMPure
AMPure
XP XP
160160
140140
120120
AMPure
AMPure
XP XP
KAPA
KAPA
PurePure
KAPA
KAPA
PurePure
AMPure
AMPure
XP XP
KAPA
KAPA
PurePure
AMPure
AMPure
XP XP
AMPure
AMPure
XP XP
Fluorescence
Fluorescence
Fluorescence
Fluorescence
120120
100100
100100
80 80
80 80
60 60
60 60
40 40
40 40
20 20
20 20
0 0
0 0
-20-20
-20-20
35 35100100200200300300
400400 600600
1000
1000
10380
10380
bp bp
Unless otherwise stated in references, presented data on file
For Research Use Only. Not for use in diagnostic procedures.
35 35100100200200300300
400400 600600
1000
1000
bp bp
10380
10380
A) Libraries were prepared with the KAPA
HyperPlus Kit, from 100 ng high-quality E. coli
genomic DNA, fragmented at 37˚C for 30 min.
to target a final library size of 300 bp.
B) Libraries were prepared with the KAPA Stranded
RNA-Seq Kit with RiboErase, from 100 ng of
Universal Human Reference (UHR) RNA according
to standard protocol.
Yields were measured with the KAPA Library
Quantification Kit post ligation. Electropherograms
for all libraries were generated with a Bioanalyzer
2100 High Sensitivity DNA Kit.
Tunable and Highly Reproducible Size Selection
• Obtain consistent library size distributions
• Flexible implementation at various points during library construction
• Adjustable size selection parameters to achieve desired library sizes
A
B
Size selected after amplification
(0.6X – 0.8X)
Amplified Library
(no size selection)
600
Size selected after amplification
(0.6X – 0.8X)
800
400
Amplified Library
(no size selection)
600
200
400
0
10
50
100 150180
300
500
1000 2000
Size selected after amplification
(0.6X – 0.8X)
800
Fluorescence
Fluorescence
Fluorescence
Fluorescence
800
10000
Amplified Library
(no size selection)
600
Size selected after amplification
(0.6X – 0.8X)
800
400
Amplified Library
(no size selection)
600
200
400
0
10
50
100 150180
300
500
1000 2000
10000
200
bp
bp
Highly reproducible final library size distribution is achieved with KAPA Pure Beads. All libraries were prepared with the KAPA Hyper Prep Kit, from 1 µg
high-quality
E. coli genomic DNA, fragmented with a Covaris® E220 Focused Ultrasonicator
using conditions optimized for mode fragment lengths of
0
2000
160
10 50 (0.6X
100 150
300
500 KAPA
1000 2000
10000 (A) or Agencourt® AMPure® XP
10(B)
50was
100performed
150180
300
500
1000 2000
10000
350 – 450 bp. Size selection
–180
0.8X) using
either
Pure Beads
after library
amplification
bp
140 Sensitivity Assay.
(2 cycles). Electropherograms were generatedbp
with a PerkinElmer® LabChip GX DNA High
200
200
Fragmented DNA
(no size selection)
Size selected after
fragmentation
(0.6X–0.8X)
Fragmented DNA
(no size selection)
Size selected
ligation
Size after
selected
after
(0.5X–0.8X)
fragmentation
(0.6X–0.8X)
100
150
D
Fluorescence
Fluorescence
C
Fluorescence
Fluorescence
150
50
Size selected
after ligation
(0.5X–0.8X)
100
0
50
120
160
100
140
80
Amplified library
(no size selection)
120
60
0.5X – 0.7X
100
40
0.6X – 0.8X
Amplified library
0.7X
– 0.9X
(no size
selection)
80
20
0.5X – 0.7X
60
0
0.6X – 0.8X
40
35
100 150 200
300
400 500 600
1000 2000
35
10380
0
300
0.7X – 0.9X
20
bp
100 150 200
400
500 600
1000 2000
10380
500 600
1000 2000
10380
bp
0
35
100 150 200
300
400 500 600
1000 2000
10380
35
100 150 200
300
400
bp
bp
Adjustable size selection at various points in library preparation. (C) Equivalent final library size is achieved by performing size selection either immediately after
genomic DNA fragmentation or after adapter-ligation. (D) Various final library sizes are achieved by timing size selection parameters after adapter-ligation. All
libraries were prepared with the KAPA Hyper Prep Kit, from 100 ng high-quality human genomic DNA, fragmented with a Covaris E220 Focused Ultrasonicator
using conditions optimized for mode fragment lengths of 350 – 450 bp. Electropherograms were generated with a Bioanalyzer 2100 High Sensitivity DNA Kit.
Ordering Information
Roche Cat. No.
Kapa Code
Description
Kit Size
07983271001
KK8000
KAPA Pure Beads
5 mL
07983280001
KK8001
KAPA Pure Beads
30 mL
07983298001
KK8002
KAPA Pure Beads
60 mL
@KapaBiosystems
Headquarters, United States
Wilmington, Massachusetts
Tel: 781.497.2933
Fax: 781.497.2934
[email protected]
KapaBiosystems
KapaBiosystems
International Office
Cape Town, South Africa
Tel: +27.21.448.8200
Fax: +27.21.448.6503
[email protected]
Kapa Technical Support
kapabiosystems.com/support
Unless otherwise stated in references, presented data on file
© 2016 Kapa Biosystems. All trademarks are the property of their respective owners.
For Research Use Only. Not for use in diagnostic procedures.
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