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Download The Treatment and Prevention of Dental Caries
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By: Scott Karas Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses What are they? ◦ Cavities How are they formed? ◦ Streptococci mutans How to prevent them? ◦ Brush your teeth ◦ Vaccine? GTF- Glucosiltransferase GbpB- Glucan binding protein B CAT- Catalytic Domain ◦ GTF epitope GLU- Glucan binding domain SYI- peptide complex ◦ GbpB epitope WIS-Water insoluble glucan Purpose of this experiment Bacteria used ◦ Streptococci mutans ◦ Streptococci sobrinus Synthetic peptides Three experiments ◦ 24-37 day old rats Measured the levels of antibodies Measured dental caries Peptides ◦ Three monoepitopic ◦ Two diepitopic Proteins ◦ GbpB from S. mutans ◦ GTP from S. sobrinus ELISA ◦ Measured IgA and IgG antibody levels Experiment 1 ◦ 40 female rats ◦ 6 groups of 7 ◦ Injected subconsciously Experiment 2- S. mutans ◦ ◦ ◦ ◦ 48 female rats 4 groups of 12 in a cage Injected subconsciously Asphyxiated Cleaned with beetles and ethanol Experiment 3- S. sobrinus ◦ Identical to Experiment 2 ◦ Streptomycin-resistant S. sobrinus Bacterial Recovery ◦ ELISA ◦ Cotton swab Carie assessment Human Trials ◦ Put in cows milk or eggs Treatment for children or infants ◦ “Window of Infectivity” Treat young mothers ◦ Breast feeding Dental Caries Methods Results Potential Uses Dental Caries Key Enzymes/Antibodies Experiment Overview Methods Results Discussion Potential Uses Smith, Daniel J., William F. King, Joy Rivero, and Martin A. Taubman. "Immunological and Protective Effects of Diepitopic Subunit Dental Caries Vaccines." Infection and Immunity 73.5 (2005): 2797-804. Print. Russell, Michael W., Noel K. Childers, Suzanne M. Michalek, Daniel J. Smith, and Martin A. Taubman. "A Caries Vaccine?" ProQuest Nursing & Allied Health Source 38.3 (2004): 230-35. Print.
