Amino acid coding of restriction enzyme recognition Download

Transcript
Amino acid coding of restriction enzyme recognition sites
numbers indicate the reading frames, the list is provided by NEB (version from 2000, i.e. a few enzymes are missing)
reformatted by Kristian M. Müller
GCN
Ala
TTR
CGN AAY GAY TGY CAR GAR GGN CAY ATH
AAR ATG TTY
CTN
Arg Asn Asp Cys Gln Glu Gly His
Ile
Lys Met Phe
Leu
Aat II
GACGT/C
3
1
Acc I
GT/MKAC
3
1
Acc65 I
G/GTACC
Afl II
C/TTAAG
Afl III
A/CRYGT
Age I
A/CCGGT
Apa I
GGGCC/C
3
ApaL I
G/TGCAC
3
Apo I
R/AATTY
Asc I
GG/CGCGCC
Ase I
AT/TAAT
Ava I
C/YCGRG
3
Avr II
C/CTAGG
1
BamH I
G/GATCC
Ban I
G/GYRCC
1,2
Ban II
GRGCY/C
3
Bbs I
GAAGAC(2/6)
Bbs I
(6/2)GTCTTC
3
3
1
1,2
1
2
1,2,3
1,2
1,2*
3
1
1
1
1
1
2
1,2
1
1
3
2
1
3,2*
1
2*
2*
1
3
1,2
2*
2
1
1,2
1,3
2
1
1
2
2*
1,2*
1
3
1
1*
1
1
2*
1
2,3
3
2
3
2
1
3
2
3
1
2
2*
1
2
3
AGY
TAR
ACN TGG TAY GTN
TCN
TGA
Thr Trp Tyr Val
Ser
Stop
2*
1,2
3
CCN
Pro
3
2*
3
2
3
1
2*
1
1
1,2*
1
2
2,2*
2
3
3
2*
1
2,2*
1
GCN
Ala
TTR
CGN AAY GAY TGY CAR GAR GGN CAY ATH
AAR ATG TTY
CTN
Arg Asn Asp Cys Gln Glu Gly His
Ile
Lys Met Phe
Leu
Bcl I
T/GATCA
Bgl II
A/GATCT
Blp I
GC/TNAGC
Bpm I
CTGGAG(16/14)
Bpm I
(14/16)CTCCAG
Bsa I
GGTCTC(1/5)
Bsa I
(5/1)GAGACC
BsaH I
GR/CGYC
BsaW I
W/CCGGW
3
BseR I
GAGGAG(10/8)
2
BseR I
(8/10)CTCCTC
Bsg I
GTGCAG(16/14)
3
2
Bsg I
(14/16)CTGCAC
3
2
BsiE I
CGRY/CG
3
BsiHKA I
GWGCW/C
3
BsiW I
C/GTACG
Bsm I
GAATGC(1/-1)
2*
Bsm I
(-1/1)GCATTC
1
BsmB I
CGTCTC(1/5)
1
BsmB I
(5/1)GAGACG
2,2*
3
BspD I
AT/CGAT
3
1
1
2
3
2
3
1,3*
1
1
1
1,2
3
1
1
1
2,3
3
1
2*
1
2*
2*
2
1
1
2
1
1
2*
3
2,2*
3
1
1
1
2
2
2
2
1
1
1,2*
2
2,3*
1
1
1,3
2
1
1
3
2*
1
1
1
2
AGY
TAR
ACN TGG TAY GTN
TCN
TGA
Thr Trp Tyr Val
Ser
Stop
1
2,3
3
CCN
Pro
1
1
2*
3
1
1
1
1
3
3
2,2*
2*
1
1,2*
1
2
1
1
3
2
3
2
3
1
1
2*
2*,3
1
2
1
1
2
GCN
Ala
TTR
CGN AAY GAY TGY CAR GAR GGN CAY ATH
AAR ATG TTY
CTN
Arg Asn Asp Cys Gln Glu Gly His
Ile
Lys Met Phe
Leu
3
1
CCN
Pro
BspE I
T/CCGGA
2
BspH I
T/CATGA
BspM I
ACCTGC(4/8)
2*
BspM I
(8/4)GCAGGT
1
Bsp1286 I
GDGCH/C
3
BsrB I
GAG/CGG
3
1
BsrF I
R/CCGGY
1,2*
3
BssH II
G/CGCGC
1,2*,3
1,2
BssS I
C/TCGTG
3
BssS I
(10/5)CACGAG
3
1
BstB I
TT/CGAA
3
1
BstE II
G/GTNACC
BstY I
R/GATCY
BstZ17 I
GTA/TAC
Bsu36 I
CC/TNAGG
2
Cla I
AT/CGAT
3
Dra I
TTT/AAA
Eae I
Y/GGCCR
3
1,2*
2
1
Eag I
C/GGCCG
3
1,2*
2
1
Ear I
CTCTTC(1/4)
Ear I
(4/1)GAAGAG
2
3
1
3
2
1
2
1
1
1,2
1
2*
2*
1
1
1,2*
2,2*
2
2
1
1
3
3*
1
3
3*
3
2*
2*
1
2,3
1
1
1
1
1,3
1
1
2
1
2
2*
1,2
1
3
3
1,3
2
2
3
2*
2
3
1
1
2
1
1
1
2
1
1
2*
2
1
1
1
1
1
1
1
1
3
2
AGY
TAR
ACN TGG TAY GTN
TCN
TGA
Thr Trp Tyr Val
Ser
Stop
1
1
2
3
1
2,2*
GCN
Ala
1,3
TTR
CGN AAY GAY TGY CAR GAR GGN CAY ATH
AAR ATG TTY
CTN
Arg Asn Asp Cys Gln Glu Gly His
Ile
Lys Met Phe
Leu
EcoO109 I
RG/GNCCY
1
EcoR I
G/AATTC
EcoR V
GAT/ATC
Fsp I
TGC/GCA
1,2
3
Hae II
RGCGC/Y
1,2
3
Hinc II
GTY/RAC
Hind III
A/AGCTT
Hpa I
GTT/AAC
Kas I
G/GCGCC
Kpn I
GGTAC/C
Mlu I
A/CGCGT
3
Msc I
TGG/CCA
3
Nae I
GCC/GGC
1,2*
Nar I
GG/CGCC
1,2
Nco I
C/CATGG
Nde I
CA/TATG
NgoM IV
G/CCGGC
1,2*
Nhe I
G/CTAGC
1,2*
Not I
GC/GGCCGC
1,1*
2,3
Nru I
TCG/CGA
3
1,2
Nsi I
ATGCA/T
3
3
1,2,3
2
3*
1
3
1
AGY
TAR
ACN TGG TAY GTN
TCN
TGA
Thr Trp Tyr Val
Ser
Stop
1
1
3
2*
1,2
2*
3
1
1
1
1
2*
3
2*
2
3
1
1
1,2
1,2,3*
3
1
CCN
Pro
1
2
1
1
3
2*
1
3
2
2
3
2*
1
2*
1
2*
1
1,2
3
1
2
1
3
1
2
3
1
2*
2*
2
1
3
3
2
1
1
3
2
2
1
3
2
1
2
2*
1
1
1
2
1
1
3
GCN
Ala
TTR
CGN AAY GAY TGY CAR GAR GGN CAY ATH
AAR ATG TTY
CTN
Arg Asn Asp Cys Gln Glu Gly His
Ile
Lys Met Phe
Leu
Pac I
TTAAT/TAA
3
PaeR7 I
C/TCGAG
Pme I
GTTT/AAAC
Pml I
CAC/GTG
3
PpuM I
RG/GWCCY
1
Psp1406 I
AA/CGTT
3
Pst I
CTGCA/G
Pvu I
CGAT/CG
Pvu II
CAG/CTG
Rsr II
CG/GWCCG
Sac I
GAGCT/C
3
Sac II
CCGC/GG
3
Sal I
G/TCGAC
Sap I
GCTCTTC(1/4)
1
Sap I
(4/1)GAAGAGC
3*
Sca I
AGT/ACT
Sfc I
C/TRYAG
Sma I
CCC/GGG
3
SnaB I
TAC/GTA
3
Spe I
A/CTAGT
Sph I
GCATG/C
1
3
1
3
3
1,2
2
2
2
3*
2,3*
1
3
1
2
1
1,2
1
2*
1
3
2
1
1
1
3
2*
1
1
1
2
1,2
1
2
3,3*
2
2
1
3
1
1
1,2*
1
3
2
3
2
3
2
1
1
1
1,2
2
1
1
2,2*
2*
1,2*
2
1
2
2
2
1
1
1
3
3
1*
1
2
3
3
1
1
2
3
2,3
1
3
1,3*
AGY
TAR
ACN TGG TAY GTN
TCN
TGA
Thr Trp Tyr Val
Ser
Stop
1,2
1
3
1,2*
CCN
Pro
2
1
3
1
2*
3
GCN
Ala
TTR
CGN AAY GAY TGY CAR GAR GGN CAY ATH
AAR ATG TTY
CTN
Arg Asn Asp Cys Gln Glu Gly His
Ile
Lys Met Phe
Leu
Ssp I
AAT/ATT
1
Stu I
AGG/CCT
Sty I
C/CWWGG
1
Xba I
T/CTAGA
1
Xho I
C/TCGAG
3
Xma I
C/CCGGG
3
3
CCN
Pro
AGY
TAR
ACN TGG TAY GTN
TCN
TGA
Thr Trp Tyr Val
Ser
Stop
1,2
1
2
2
2*
1
1,2*
3
2*
2
2,3
1
3
3
1
1
2
1
1
2
1,2
3
3
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