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Antibiotics
used as a selective agent in the molecular genetic
research. Biochrom AG offers G 418-BC in powder
form and as a sterile ready-to-use preparation.
A 291-25
10 g
A 2912
50 ml
Ultra pure
water
G 418-BC, liquid (ready-to-use solution)
sterile, activity 30000 U/ml (50 mg/ml),
in distilled water; dilute according to application
Storage temperature: +2 – +8 °C
Unit
Buffers and
solutions
G 418-BC, crystalline
not sterile (G 418 sulfate), activity > 500 U/mg;
to be dissolved in distilled water and sterile filtered
Storage temperature: +2 – +8 °C
Cat. No.
Separating
solutions
Product
Media
G 418-BC is an antibiotic belonging to the aminoglycosides group. Due to its toxicity on bacteria,
fungi, protozoa, and mammalian cells it is widely
Sera
G 418-BC: Selective antibiotic for molecular research and gene transfer
Antibiotics
Enzymes
Dosage:
Cell culture
reagents
The required dose of G 418-BC for the selection of resistant cells varies in dependence of the cell type and the current
phase of the cell cycle. Thereby a cell in the interphase (“resting phase”) of G 418-BC is less affected than in the mitosis
(“separating phase”). But also on separating-active cells, the cells’ death occurs only after 3 to 7 days. The recommended
concentration at G 418-BC in the medium has for eukaryotic cells 400 mg/l (for the selection) or 200 mg/l (for conservation).
Cell culture
diagnostics
Cytokines and
growth factors
Mycoplasma
reagents
Cell attachment factors
Industrial
cell culture
More Information Under www.biochrom.de
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