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As consequence of change of consumer eating habits, international travel and food market globalization we assist to increase of parasitological disease. Anisakis is a nematode worm wich could be ingest with raw fi sh. Diphyllobotrium is an elmintic worm wich could infect humans, pigs, cats, dogs. Entamoeba is a human parasite wich is the second death cause for parasitism. It is very important to search parasite into food in order to limit worldwide diffusion of parasite and to protect consumer health. In this fi eld molecular biology methods seem to be the faster techniques to search parasite