BT314 Virology
... version and one defective version of the gene; the normal version produces enough of the clotting factor to prevent hemophilia. A female exhibits hemophilia only if she inherits two defective copies of the gene, which is rare. Because males have a single X chromosome per cell, if they inherit a defe ...
... version and one defective version of the gene; the normal version produces enough of the clotting factor to prevent hemophilia. A female exhibits hemophilia only if she inherits two defective copies of the gene, which is rare. Because males have a single X chromosome per cell, if they inherit a defe ...
Introduction to Genetics
... version and one defective version of the gene; the normal version produces enough of the clotting factor to prevent hemophilia. A female exhibits hemophilia only if she inherits two defective copies of the gene, which is rare. Because males have a single X chromosome per cell, if they inherit a defe ...
... version and one defective version of the gene; the normal version produces enough of the clotting factor to prevent hemophilia. A female exhibits hemophilia only if she inherits two defective copies of the gene, which is rare. Because males have a single X chromosome per cell, if they inherit a defe ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... • Traditional DNA intercalating markers such as ethidium bromide have been replaced by less toxic substitutes such as GelRed, which is highly similar to EtBr in structure • Rough estimates of product concentration can also be made by comparison with the brightness of products of known DNA quanty, su ...
... • Traditional DNA intercalating markers such as ethidium bromide have been replaced by less toxic substitutes such as GelRed, which is highly similar to EtBr in structure • Rough estimates of product concentration can also be made by comparison with the brightness of products of known DNA quanty, su ...
Nerve activates contraction
... Karl Correns in 1909 first observed cytoplasmic genes in plants. He determined that the coloration of the offspring was determined only by the maternal parent. These coloration patterns are due to genes in the plastids which are inherited only via the ovum, not the pollen. ...
... Karl Correns in 1909 first observed cytoplasmic genes in plants. He determined that the coloration of the offspring was determined only by the maternal parent. These coloration patterns are due to genes in the plastids which are inherited only via the ovum, not the pollen. ...
Document
... BIO 6: Effects of genetic recombination and mutation; and BIO 8: Investigating how variation of traits, reproductive strategies and environmental pressures impact on the survival of populations. ...
... BIO 6: Effects of genetic recombination and mutation; and BIO 8: Investigating how variation of traits, reproductive strategies and environmental pressures impact on the survival of populations. ...
Universal chloroplast integration and expression vectors
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
... The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants ...
Fanconi anemia and RAD50 deficiency: genetic and functional
... The first of these diseases is Fanconi anemia (FA). Several FA genes have been identified in the last few years, raising questions regarding cellular phenotype, mutation spectra, frequency, function , and genotype-phenotype correlations. At the beginning of this thesis only eight of the currently tw ...
... The first of these diseases is Fanconi anemia (FA). Several FA genes have been identified in the last few years, raising questions regarding cellular phenotype, mutation spectra, frequency, function , and genotype-phenotype correlations. At the beginning of this thesis only eight of the currently tw ...
paper - Université de Namur
... a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and further applications to other helical molecules). In section VI, the most important historical X-ray pictures of DNA fibres and their meaning for the structure of the molecule are described ...
... a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and further applications to other helical molecules). In section VI, the most important historical X-ray pictures of DNA fibres and their meaning for the structure of the molecule are described ...
Rethinking heredity, again
... molecular genetics, the exclusive one-way passage of information from DNA sequence to RNA to protein [20]. Consequently, the possibility that an individual’s experiences during its lifetime could have predictable effects on the phenotype of its offspring was deemed to be conclusively refuted and res ...
... molecular genetics, the exclusive one-way passage of information from DNA sequence to RNA to protein [20]. Consequently, the possibility that an individual’s experiences during its lifetime could have predictable effects on the phenotype of its offspring was deemed to be conclusively refuted and res ...
Rethinking heredity, again
... molecular genetics, the exclusive one-way passage of information from DNA sequence to RNA to protein [20]. Consequently, the possibility that an individual’s experiences during its lifetime could have predictable effects on the phenotype of its offspring was deemed to be conclusively refuted and res ...
... molecular genetics, the exclusive one-way passage of information from DNA sequence to RNA to protein [20]. Consequently, the possibility that an individual’s experiences during its lifetime could have predictable effects on the phenotype of its offspring was deemed to be conclusively refuted and res ...
Single-stranded heteroduplex intermediates in l Red homologous
... DNA. A stretch of heterologous dsDNA, which usually contains a selectable gene, is introduced into the middle of the copy. The incorporation of the linear dsDNA by HR incorporates the central heterologous sequence and so alters the target by ‘replacement’. This application of HR differs from endogen ...
... DNA. A stretch of heterologous dsDNA, which usually contains a selectable gene, is introduced into the middle of the copy. The incorporation of the linear dsDNA by HR incorporates the central heterologous sequence and so alters the target by ‘replacement’. This application of HR differs from endogen ...
Centromere Locations and Associated Chromosome
... CEN5 is more complex. The formation of chromosome V of A. thaliana involved a reciprocal translocation (see above and Figure 1), and an understanding of this event is critical for interpreting the origin of CEN5. Previous comparative molecular cytogenetic analyses of A. thaliana and A. lyrata could ...
... CEN5 is more complex. The formation of chromosome V of A. thaliana involved a reciprocal translocation (see above and Figure 1), and an understanding of this event is critical for interpreting the origin of CEN5. Previous comparative molecular cytogenetic analyses of A. thaliana and A. lyrata could ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... Construction of pEF1α-GFP. A DNA fragment comprising the hrGFP gene was PCR amplified from PSXrabpA (Stratagene) with primers attB1-WdV020 and attB2-WdV021. The hrGFP was recombined into pEF5/FRT/V5-DEST using the Gateway technology, resulting in the hrGFP gene expression vector pHY01. The EF1α prom ...
... Construction of pEF1α-GFP. A DNA fragment comprising the hrGFP gene was PCR amplified from PSXrabpA (Stratagene) with primers attB1-WdV020 and attB2-WdV021. The hrGFP was recombined into pEF5/FRT/V5-DEST using the Gateway technology, resulting in the hrGFP gene expression vector pHY01. The EF1α prom ...
Biology II Final Exam Practice
... ____ 12. Natural selection is based on the concepts of excess reproduction, variation, inheritance, and the advantages of certain traits. ____ 13. Darwin developed his theory of evolution exclusively from his work on the Galapagos Islands. ____ 14. Fossils, although interesting, do not actually prov ...
... ____ 12. Natural selection is based on the concepts of excess reproduction, variation, inheritance, and the advantages of certain traits. ____ 13. Darwin developed his theory of evolution exclusively from his work on the Galapagos Islands. ____ 14. Fossils, although interesting, do not actually prov ...
genomebiology.com
... Deeper analysis of the non-mammalian genomes revealed that TEs are less likely to be fixed within transcribed regions relative to orthologous regions in human and mouse [16]. In G. gallus, D. rerio and C. intestinalis, 33.2%, 47.3% and 39.4% of TEs reside within introns, respectively, whereas in the ...
... Deeper analysis of the non-mammalian genomes revealed that TEs are less likely to be fixed within transcribed regions relative to orthologous regions in human and mouse [16]. In G. gallus, D. rerio and C. intestinalis, 33.2%, 47.3% and 39.4% of TEs reside within introns, respectively, whereas in the ...
Complete genome sequence of the thermophilic Thermus sp
... between 47 and 48. In terms of transposase genes, T. scotoductus SA-01 has the highest number (22 genes), followed by CCB_US3_UF1 (13 genes), T. thermophilus HB27 (18 genes), and HB8 (18 genes). Interestingly, no prophage-related genes are found in these four genomes, implying the occurrence of clus ...
... between 47 and 48. In terms of transposase genes, T. scotoductus SA-01 has the highest number (22 genes), followed by CCB_US3_UF1 (13 genes), T. thermophilus HB27 (18 genes), and HB8 (18 genes). Interestingly, no prophage-related genes are found in these four genomes, implying the occurrence of clus ...
Evolution of mating types driven by purifying selection
... develop the ability to recognize a universal mitochondrial marker protein, at the same time protecting its own organelles from degradation, e.g. by ceasing the expression of the marker in its own mitochondria (allele Um, Fig. 1b). I term this mechanism the “maternal” mode of UPI, and the gamete dest ...
... develop the ability to recognize a universal mitochondrial marker protein, at the same time protecting its own organelles from degradation, e.g. by ceasing the expression of the marker in its own mitochondria (allele Um, Fig. 1b). I term this mechanism the “maternal” mode of UPI, and the gamete dest ...
Pourcel et al., Microbiology 2005
... 91001 (Zhou et al., 2004) was achieved using the tool described by Denoeud & Vergnaud (2004) and accessible at http://minisatellites. u-psud.fr/blast/blast_primers_multi.htm. The corresponding new MLVA pattern is represented by genotype 62. ...
... 91001 (Zhou et al., 2004) was achieved using the tool described by Denoeud & Vergnaud (2004) and accessible at http://minisatellites. u-psud.fr/blast/blast_primers_multi.htm. The corresponding new MLVA pattern is represented by genotype 62. ...
Directionality in FLP Protein-promoted Site
... sites of all systems studied to datecontain elements of asym- Dideoxy I n Vitro FLP Reactions-Reactions were carried out essentially as metry which define orientation. Reactions between two sites described previously (4).Reactions (20 pl) contained 25 mM Trison the same DNA molecule alwaysresult in ...
... sites of all systems studied to datecontain elements of asym- Dideoxy I n Vitro FLP Reactions-Reactions were carried out essentially as metry which define orientation. Reactions between two sites described previously (4).Reactions (20 pl) contained 25 mM Trison the same DNA molecule alwaysresult in ...
... embryos. 5meC lane = DNA precipitated by antibody against methylated cytosine; IgG = non-specific immunoprecipitation; Input = DNA before immunoprecipitation; - = no antibody control. Specific bands for Kcnq1 and Kcnq1ot1 are indicated; NS = non-specific amplification product. The Kcnq1ot1 promoter ...
1q21 microdeletions
... segments. To pair up precisely, each chromosome ‘recognises’ matching or nearmatching DNA sequences on its partner chromosome. However, throughout the chromosomes there are many DNA sequences that are so similar that it is thought that mispairing can occur. The 1q21.1 region has an extremely complex ...
... segments. To pair up precisely, each chromosome ‘recognises’ matching or nearmatching DNA sequences on its partner chromosome. However, throughout the chromosomes there are many DNA sequences that are so similar that it is thought that mispairing can occur. The 1q21.1 region has an extremely complex ...
1q21 microdeletions
... million base pairs, or one so-called megabase (Mb) of DNA. Base pairs are the chemicals in DNA that form the ends of the ‘rungs’ of its ladder-like structure. Chromosome 1 has around 247 Mb in total and band 1q21.1 alone contains around 5.4 Mb. In a class 1 deletion, DNA is typically missing between ...
... million base pairs, or one so-called megabase (Mb) of DNA. Base pairs are the chemicals in DNA that form the ends of the ‘rungs’ of its ladder-like structure. Chromosome 1 has around 247 Mb in total and band 1q21.1 alone contains around 5.4 Mb. In a class 1 deletion, DNA is typically missing between ...
Extrachromosomal DNA
Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.