Raines, ChemRev 1998
... cleft (Figure 172). The predominant elements of secondary structure are a long four-stranded antiparallel β-sheet and three short R-helixes. The enzyme is cross-linked by four disulfide bonds, which involve all eight of its cysteine residues. The peptide bonds preceding two of the four proline resid ...
... cleft (Figure 172). The predominant elements of secondary structure are a long four-stranded antiparallel β-sheet and three short R-helixes. The enzyme is cross-linked by four disulfide bonds, which involve all eight of its cysteine residues. The peptide bonds preceding two of the four proline resid ...
Euglena gracilis ascorbate peroxidase forms an intramolecular
... 649 amino acid residues (see Supplementary Figure S1 available at http://www.BiochemJ.org/bj/426/bj4260125add.htm). The cDNA obtained had an exceptionally high GC content of 70.8 %. The N-terminal and internal peptide sequences that were identified previously in the purified Euglena APX could be fou ...
... 649 amino acid residues (see Supplementary Figure S1 available at http://www.BiochemJ.org/bj/426/bj4260125add.htm). The cDNA obtained had an exceptionally high GC content of 70.8 %. The N-terminal and internal peptide sequences that were identified previously in the purified Euglena APX could be fou ...
Metabolism of [14C]glutamate and [14C]glutamine by
... [14C]glutamineaccounted for 25 'YOof the radioactivity in the amino acid pool (Fig. l a ) while [14C]glutamate represented 51 YOof the radioactivity. These proportions did not vary greatly during the 2 h feeding period. The label was also detected in a range of amino acids including aspartate, aspar ...
... [14C]glutamineaccounted for 25 'YOof the radioactivity in the amino acid pool (Fig. l a ) while [14C]glutamate represented 51 YOof the radioactivity. These proportions did not vary greatly during the 2 h feeding period. The label was also detected in a range of amino acids including aspartate, aspar ...
animal nutrition - e
... Figure 1.1 after Response in the Yield of Milk Constituents to the Intake of Nutrients by Dairy Cows. AFRC Technical Committee on Responses to Nutrients, Report No. 11, CABI Publishing International,Wallingford (1998); Figure 5.1 adapted from Roche Vitec Animal Nutrition and Vitamin News, Vol. 1, A1 ...
... Figure 1.1 after Response in the Yield of Milk Constituents to the Intake of Nutrients by Dairy Cows. AFRC Technical Committee on Responses to Nutrients, Report No. 11, CABI Publishing International,Wallingford (1998); Figure 5.1 adapted from Roche Vitec Animal Nutrition and Vitamin News, Vol. 1, A1 ...
Overview of relaxin
... synthesis and maintainence of collagen, our primary connective tissue protein. Every part of our body and virtually every body function is thus affected or potentially influenced by relaxin. Muscle/tendon and ligament integrity, nerve conductivity, heart beat regularity, and even bone health all are ...
... synthesis and maintainence of collagen, our primary connective tissue protein. Every part of our body and virtually every body function is thus affected or potentially influenced by relaxin. Muscle/tendon and ligament integrity, nerve conductivity, heart beat regularity, and even bone health all are ...
Forced Expression of Dystrophin Deletion Constructs Reveals
... (Fig. 3). These mosaic-expressing mice, however, were able to be analyzed for proper localization of the members of the D A P complex. To establish that formation of the dystroglycan complex is independent of uniform dystrophin expression, we analyzed the pattern of [3-dystroglycan localization in a ...
... (Fig. 3). These mosaic-expressing mice, however, were able to be analyzed for proper localization of the members of the D A P complex. To establish that formation of the dystroglycan complex is independent of uniform dystrophin expression, we analyzed the pattern of [3-dystroglycan localization in a ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
... diagnostic arena due to a number of factors. One is the enzyme’s limited stability under the prevalent conditions, including elevated temperatures and in the presence of hydrogen peroxide. We are using methods of directed evolution (Morawski et al., 2000; Cherry et al., 1999; Petrounia and Arnold, 2 ...
... diagnostic arena due to a number of factors. One is the enzyme’s limited stability under the prevalent conditions, including elevated temperatures and in the presence of hydrogen peroxide. We are using methods of directed evolution (Morawski et al., 2000; Cherry et al., 1999; Petrounia and Arnold, 2 ...
Amino Acids in Maturation Medium and Presence
... with fetal calf serum (FCS) [15, 18]. In the present study using simple medium supplemented with PFF and gonadotropins, most oocytes reached M-II also by 36 h of culture. These results may indicate that FCS, PFF, and gonadotropins contain the factors that regulate oocyte maturation. The maturation r ...
... with fetal calf serum (FCS) [15, 18]. In the present study using simple medium supplemented with PFF and gonadotropins, most oocytes reached M-II also by 36 h of culture. These results may indicate that FCS, PFF, and gonadotropins contain the factors that regulate oocyte maturation. The maturation r ...
ldentification of Surface-Exposed Domains on the Reducing Side of
... and FB(Oh-oka et al., 1988). PsaD provides an essential Fddocking site on the reducing side of PSI (Zanetti and Merati, 1987; Zilber and Malkin, 1988; Lelong et al., 1994; Xu et al., 1994a) and is also required for the stable assembly of PsaC and PsaE into the PSI complex (Li et al., 1991; Chitnis a ...
... and FB(Oh-oka et al., 1988). PsaD provides an essential Fddocking site on the reducing side of PSI (Zanetti and Merati, 1987; Zilber and Malkin, 1988; Lelong et al., 1994; Xu et al., 1994a) and is also required for the stable assembly of PsaC and PsaE into the PSI complex (Li et al., 1991; Chitnis a ...
The effect of higher protein human milk fortifier on growth in preterm
... adult metabolic health outcomes than adipose tissue gain in the in-hospital period, was also assessed weekly. Blood and urine chemistry markers were assessed weekly and every two weeks, respectively, as an assessment of protein nutritional status. A weekly sample of breast milk was collected if supp ...
... adult metabolic health outcomes than adipose tissue gain in the in-hospital period, was also assessed weekly. Blood and urine chemistry markers were assessed weekly and every two weeks, respectively, as an assessment of protein nutritional status. A weekly sample of breast milk was collected if supp ...
Flux analysis in central carbon metabolism in plants
... A concept called 'bondomer' was introduced, to simplify flux evaluation from labeling experiments that employ U-13C (uniformly 13C-labeled) substrates. It was shown that the enumeration of feasible bondomers in a metabolic network reduced the number of bondomer balances 3-fold. This resulted in a si ...
... A concept called 'bondomer' was introduced, to simplify flux evaluation from labeling experiments that employ U-13C (uniformly 13C-labeled) substrates. It was shown that the enumeration of feasible bondomers in a metabolic network reduced the number of bondomer balances 3-fold. This resulted in a si ...
Regulation of Phenylalanine and Tyrosine
... were assayed as described in Methods. The Ki value of L-phenylalanine in the chorismate mutase reaction was determined at 0.9 mM-chorismate. The Ki value of L-phenylalanine in the prephenate dehydratase I reaction as well as the K, value of L-tyrosine were each determined at 0 . 3 4 m ~ prephenate. ...
... were assayed as described in Methods. The Ki value of L-phenylalanine in the chorismate mutase reaction was determined at 0.9 mM-chorismate. The Ki value of L-phenylalanine in the prephenate dehydratase I reaction as well as the K, value of L-tyrosine were each determined at 0 . 3 4 m ~ prephenate. ...
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... et al., 1988) the first two, but not the third, are necessary for activin A binding (Keutmann et al., 2004; Harrington et al., 2006). Aside from activins, follistatin also binds several bone morphogenetic proteins (BMP) including BMP2, BMP4, BMP6 and BMP7 (Iemura et al., 1998; Glister et al., 2004). ...
... et al., 1988) the first two, but not the third, are necessary for activin A binding (Keutmann et al., 2004; Harrington et al., 2006). Aside from activins, follistatin also binds several bone morphogenetic proteins (BMP) including BMP2, BMP4, BMP6 and BMP7 (Iemura et al., 1998; Glister et al., 2004). ...
PATRICK PROBLEMS 2 Key
... free base but not when it is ionized. Further details on the binding interactions of noradrenaline and adrenaline can be found in sections 15.14.1, 20.8 and 20.9 ...
... free base but not when it is ionized. Further details on the binding interactions of noradrenaline and adrenaline can be found in sections 15.14.1, 20.8 and 20.9 ...
The Plant Cell - Department of Biology
... Figure 2. Amino Acid Sequence Comparison and Phylogenetic Analysis of KIC and Other Related Proteins Containing EF-Hand Motifs. (A) Sequence alignment of KIC, KRPs, and other Ca2 binding proteins. Amino acids that are identical to the KIC sequence are indicated by white letters on a black backgroun ...
... Figure 2. Amino Acid Sequence Comparison and Phylogenetic Analysis of KIC and Other Related Proteins Containing EF-Hand Motifs. (A) Sequence alignment of KIC, KRPs, and other Ca2 binding proteins. Amino acids that are identical to the KIC sequence are indicated by white letters on a black backgroun ...
... The present invention relates to nucleic acid molecules related to the var2csa gene family as well as amino acid sequences encoded by such nucleic acid molecules with respect to their role in mediating adhesion of infected red blood cells to chondroitin sulphate A (CSA) in the placenta which is char ...
Supplementary materials - Stanford Artificial Intelligence Laboratory
... this distribution is encoded using a mean and variance stored at the corresponding leaf. The model semantics is that, given a gene G and an array A in a context, the probability of observing some expression value for gene G in an array A is governed by the normal distribution specified for that cont ...
... this distribution is encoded using a mean and variance stored at the corresponding leaf. The model semantics is that, given a gene G and an array A in a context, the probability of observing some expression value for gene G in an array A is governed by the normal distribution specified for that cont ...
Multilevel functional and structural defects induced by two
... of the absorbance against a temperature curve. The melting profiles of the transcripts were multiphasic as expected from RNAs exhibiting different levels of folding with secondary and tertiary structures. In general, a first transition was observed upon the breakdown of the tertiary structure of the ...
... of the absorbance against a temperature curve. The melting profiles of the transcripts were multiphasic as expected from RNAs exhibiting different levels of folding with secondary and tertiary structures. In general, a first transition was observed upon the breakdown of the tertiary structure of the ...
Viva Voce : Orals in Biochemistry
... 10. Chemistry and metabolism of nucleic acids. ............................................................ 83 11. (i) Molecular biology – I – A Replication ............................................................ 93 (ii) Molecular biology I – B Transcription .................................... ...
... 10. Chemistry and metabolism of nucleic acids. ............................................................ 83 11. (i) Molecular biology – I – A Replication ............................................................ 93 (ii) Molecular biology I – B Transcription .................................... ...
SSEQ
... production of bispecific diabodies could be increased from 72% by the parental diabody to over 90% by the knob-into-hole diabody. Importantly, production yields only slightly decrease as a result of these mutations. However, a reduction in antigen-binding activity was observed for several constructs ...
... production of bispecific diabodies could be increased from 72% by the parental diabody to over 90% by the knob-into-hole diabody. Importantly, production yields only slightly decrease as a result of these mutations. However, a reduction in antigen-binding activity was observed for several constructs ...
The Role of N- and C-terminal Amino Acids to
... thesis. Without his time and advice, this project would not have been possible. I would now like to thank my parents for their support throughout my seven years of postsecondary education. You were always there for me when I needed additional encouragement and I am forever grateful. Thank you to my ...
... thesis. Without his time and advice, this project would not have been possible. I would now like to thank my parents for their support throughout my seven years of postsecondary education. You were always there for me when I needed additional encouragement and I am forever grateful. Thank you to my ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... The ribosomal stalk in bacteria is composed of four or six copies of L12 proteins arranged in dimers that bind to the adjacent sites on protein L10, spanning 10 amino acids each from the L10 C-terminus. To study why multiple L12 dimers are required on the ribosome, we created a chromosomally enginee ...
... The ribosomal stalk in bacteria is composed of four or six copies of L12 proteins arranged in dimers that bind to the adjacent sites on protein L10, spanning 10 amino acids each from the L10 C-terminus. To study why multiple L12 dimers are required on the ribosome, we created a chromosomally enginee ...
on the importance of cooperativity effects - RSC Publishing
... through the Protein Data Bank (PDB) showed that anion–p close contacts exist in experimental protein structures between the standard aromatic residues (Trp, Phe, Tyr, and His) and anions, such as chloride and phosphate.25 Also, by a systematic search of protein structures followed by ab initio calcu ...
... through the Protein Data Bank (PDB) showed that anion–p close contacts exist in experimental protein structures between the standard aromatic residues (Trp, Phe, Tyr, and His) and anions, such as chloride and phosphate.25 Also, by a systematic search of protein structures followed by ab initio calcu ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.