DEVELOPMENT OF LACTIC ACID PRODUCTION
... fermentation of L-(+)-lactic acid (lactate) was carried out using the electrodeionization (EDI) technique. The effect of initial lactate concentrations on microbial growth was initially investigated. A mathematical simulation of the product inhibition was successfully illustrated. It was found that ...
... fermentation of L-(+)-lactic acid (lactate) was carried out using the electrodeionization (EDI) technique. The effect of initial lactate concentrations on microbial growth was initially investigated. A mathematical simulation of the product inhibition was successfully illustrated. It was found that ...
rapid acting insulin analogues - The Association of Physicians of India
... follow-up, there was no difference (p=0.368) in HbA1c between the 2 groups. As with lispro, the most commonly reported adverse reaction with aspart is hypoglycemia. In all published studies, it appears that the overall incidence of hypoglycemic events is similar between aspart and regular insulin. H ...
... follow-up, there was no difference (p=0.368) in HbA1c between the 2 groups. As with lispro, the most commonly reported adverse reaction with aspart is hypoglycemia. In all published studies, it appears that the overall incidence of hypoglycemic events is similar between aspart and regular insulin. H ...
The Role of N- and C-terminal Amino Acids to
... This thesis is an investigation of the role of the prosegment (PS) of pepsinogen in the binding, refolding and inhibition of pepsin. Native pepsin (Np) is irreversibly denatured, and folds to a stable, non-native state under refolding conditions, termed refolded pepsin (Rp) (Dee and Yada 2010). When ...
... This thesis is an investigation of the role of the prosegment (PS) of pepsinogen in the binding, refolding and inhibition of pepsin. Native pepsin (Np) is irreversibly denatured, and folds to a stable, non-native state under refolding conditions, termed refolded pepsin (Rp) (Dee and Yada 2010). When ...
Ch23-Oxidation of Fatty Acids and Ketone Bodies
... acids are released from adipose tissue triacylglycerols. They circulate through blood bound to albumin (Fig. 23.1). In cells, they are converted to fatty acyl CoA derivatives by acyl CoA synthetases. The activated acyl group is transported into the mitochondrial matrix bound to carnitine, where fatt ...
... acids are released from adipose tissue triacylglycerols. They circulate through blood bound to albumin (Fig. 23.1). In cells, they are converted to fatty acyl CoA derivatives by acyl CoA synthetases. The activated acyl group is transported into the mitochondrial matrix bound to carnitine, where fatt ...
The Bacterial Toxin RelE Displays Codon
... studied more systematically with RCs programmed with twenty-one mRNAs of XR7 type, which were identical except for the fifth codon of their ORFs. This was either one of the three stop codons or a sense codon that can be reached from anyone of the stop codons by a single mutation (Freistroffer et al. ...
... studied more systematically with RCs programmed with twenty-one mRNAs of XR7 type, which were identical except for the fifth codon of their ORFs. This was either one of the three stop codons or a sense codon that can be reached from anyone of the stop codons by a single mutation (Freistroffer et al. ...
Bioinformatic Analysis of Glycoside Hydrolases in the
... [14]. Representative Actinobacterial sequences from the CAZy families were used to identify homologs in the proteomes of the Actinobacteria using BLAST [15]. Domains in the glycoside hydrolase proteins were identified using the NCBI’s CDDsearch tool [16]. Amino acid substitutions between homologs of ...
... [14]. Representative Actinobacterial sequences from the CAZy families were used to identify homologs in the proteomes of the Actinobacteria using BLAST [15]. Domains in the glycoside hydrolase proteins were identified using the NCBI’s CDDsearch tool [16]. Amino acid substitutions between homologs of ...
Production of L-(+) Lactic Acid From Blackstrap Molasses by
... beautiful work on the scanning electron microscopy are shown in my dissertation. I would like to express my deeply appreciation to Ms. Durriya Sarkar at the Audubon Sugar Institute (ASI). She helped and gave me advice through my work and many other topics. Her kindness wiil never be forgotten. My gr ...
... beautiful work on the scanning electron microscopy are shown in my dissertation. I would like to express my deeply appreciation to Ms. Durriya Sarkar at the Audubon Sugar Institute (ASI). She helped and gave me advice through my work and many other topics. Her kindness wiil never be forgotten. My gr ...
c12) United States Patent - Rice Scholarship Home
... NADH. This system of cofactor regeneration has been successfully applied in the production of optically active amino acids (Galkin eta!., 1997), chiral hydroxy acids, esters, alcohols, and other fine chemicals synthesized by different dehydrogenases (Hummel and Kula, 1989), (Tishkov eta!., 1999). Pu ...
... NADH. This system of cofactor regeneration has been successfully applied in the production of optically active amino acids (Galkin eta!., 1997), chiral hydroxy acids, esters, alcohols, and other fine chemicals synthesized by different dehydrogenases (Hummel and Kula, 1989), (Tishkov eta!., 1999). Pu ...
Solid Phase Synthesis on SASRIN
... obtained with triphenylphosphine dichloride [24,25,26a] which was prepared in situ from triphenylphosphine and tetrachloromethane [26b] (SASRIN bromide could be obtained as well, by treating the resin with the dimethyl sulfide/N-bromosuccinimide complex [27]. Our own attempts to convert SASRIN into ...
... obtained with triphenylphosphine dichloride [24,25,26a] which was prepared in situ from triphenylphosphine and tetrachloromethane [26b] (SASRIN bromide could be obtained as well, by treating the resin with the dimethyl sulfide/N-bromosuccinimide complex [27]. Our own attempts to convert SASRIN into ...
Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... by centrifugation for 30 min at 4,000g to recover the ELP-ZmAb precipitates. The supernatants after centrifugation were collected and analyzed using analytical Protein A chromatography and reversed phase liquid chromatography (RPLC) (discussed below) for the presence of leftover mAb and ELP-Z, respe ...
... by centrifugation for 30 min at 4,000g to recover the ELP-ZmAb precipitates. The supernatants after centrifugation were collected and analyzed using analytical Protein A chromatography and reversed phase liquid chromatography (RPLC) (discussed below) for the presence of leftover mAb and ELP-Z, respe ...
Structure and function of Escherichia coli valine transfer RNA in
... translational process (Sprinzl and Cramer, 1979). tRNAs decode genetic information through base pairing of their anticodons with the codons of a programmed messenger RNA (mRNA) on the ribosome, and they transfer amino acids into a growing polypeptide chain through the interaction of 3'-CCA of tRNA w ...
... translational process (Sprinzl and Cramer, 1979). tRNAs decode genetic information through base pairing of their anticodons with the codons of a programmed messenger RNA (mRNA) on the ribosome, and they transfer amino acids into a growing polypeptide chain through the interaction of 3'-CCA of tRNA w ...
Amines and Amides
... 1939), who observed the antibacterial action of the red dye Protonsil; further researched showed that it was the metabolic byproduct, sulfanilamide, which was the active form. It prevents bacteria from synthesizing folic acid, which they need in order to grow. Bacterial enzymes synthesize folic acid ...
... 1939), who observed the antibacterial action of the red dye Protonsil; further researched showed that it was the metabolic byproduct, sulfanilamide, which was the active form. It prevents bacteria from synthesizing folic acid, which they need in order to grow. Bacterial enzymes synthesize folic acid ...
Acid-Base 2013 - UMF IASI 2015
... •rarely, metabolic acidosis can be part of a mixed or complex acid-base disturbance •2 or more separate metabolic or respiratory derangements occur together pH may not be reduced or the HCO3 - concentration may not be low ...
... •rarely, metabolic acidosis can be part of a mixed or complex acid-base disturbance •2 or more separate metabolic or respiratory derangements occur together pH may not be reduced or the HCO3 - concentration may not be low ...
Characterization of the Two-Component, FAD-Dependent Monooxygenase SgcC That Requires Carrier Protein-Tethered
... chromophore. The chromophore has four distinct chemical moieties, including an (S)-3-chloro-5-hydroxyβ-tyrosine moiety, the biosynthesis of which from L-R-tyrosine requires five proteins: SgcC, SgcC1, SgcC2, SgcC3, and SgcC4; a sixth protein, SgcC5, catalyzes the incorporation of this β-amino acid m ...
... chromophore. The chromophore has four distinct chemical moieties, including an (S)-3-chloro-5-hydroxyβ-tyrosine moiety, the biosynthesis of which from L-R-tyrosine requires five proteins: SgcC, SgcC1, SgcC2, SgcC3, and SgcC4; a sixth protein, SgcC5, catalyzes the incorporation of this β-amino acid m ...
TaqMan-Based Real-Time PCR for Genotyping Common
... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
Effects of low crude-protein diets fortified with crystalline amino acids
... protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from that needed by the animal because sufficient protein must be added to meet or exceed ...
... protein. When corn and SBM are used to supply all of the protein, the diet contains amino acids in excess of the pigs' or broilers' requirements. The balance of amino acids in a given corn-SBM diet is different from that needed by the animal because sufficient protein must be added to meet or exceed ...
Designing synthetic MLPA probes - MRC
... corresponding NG_sequence here: http://www.ncbi.nlm.nih.gov/refseq/rsg/browse/. N.b. NG_sequences also include intronic sequences. Nucleotide. The combination of LPO and RPO. DNA oligonucleotide which, when annealed to a complementary DNA sequence, can be used as starting point for extension by a po ...
... corresponding NG_sequence here: http://www.ncbi.nlm.nih.gov/refseq/rsg/browse/. N.b. NG_sequences also include intronic sequences. Nucleotide. The combination of LPO and RPO. DNA oligonucleotide which, when annealed to a complementary DNA sequence, can be used as starting point for extension by a po ...
Metabolism of [14C]glutamate and [14C]glutamine by
... 10.4 MBq pmol-'; New England Nuclear) or 3.7 kBq L[U-14C]glutamine (specific activity 7.77 MBq pmol-' ; New England Nuclear). At the end of the feeding period, the mycelial discs were washed for 5 min with 0.1 mM CaSO, and freezedried prior to analysis. Separation of amino acids. Amino acids were ex ...
... 10.4 MBq pmol-'; New England Nuclear) or 3.7 kBq L[U-14C]glutamine (specific activity 7.77 MBq pmol-' ; New England Nuclear). At the end of the feeding period, the mycelial discs were washed for 5 min with 0.1 mM CaSO, and freezedried prior to analysis. Separation of amino acids. Amino acids were ex ...
Analysis of the genetic potential of the spongederived fungus
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
... lab and for introducing me into the puzzling world of polyketides. I am grateful for all suggestions that I got from him during the practical work in the lab, as well as for the writing of my thesis. Moreover, I want to thank him for his trust and unique experience in building up a laboratory togeth ...
Nucleic acid vaccines for ehrlichia chaffeensis and methods of use
... cattle that have been subjected to centuries of natural selection. The problems occur Where the disease contacts susceptible or naive cattle and other ruminants. HeartWater has been con?rmed to be on the island of Guadeloupe in the ...
... cattle that have been subjected to centuries of natural selection. The problems occur Where the disease contacts susceptible or naive cattle and other ruminants. HeartWater has been con?rmed to be on the island of Guadeloupe in the ...
Nucleic acid analogue
Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research.Nucleic acids are chains of nucleotides, which are composed of three parts: a phosphate backbone, a pucker-shaped pentose sugar, either ribose or deoxyribose, and one of four nucleobases.An analogue may have any of these altered. Typically the analogue nucleobases confer, among other things, different base pairing and base stacking properties. Examples include universal bases, which can pair with all four canonical bases, and phosphate-sugar backbone analogues such as PNA, which affect the properties of the chain (PNA can even form a triple helix).Nucleic acid analogues are also called Xeno Nucleic Acid and represent one of the main pillars of xenobiology, the design of new-to-nature forms of life based on alternative biochemistries.Artificial nucleic acids include peptide nucleic acid (PNA), Morpholino and locked nucleic acid (LNA), as well as glycol nucleic acid (GNA) and threose nucleic acid (TNA). Each of these is distinguished from naturally occurring DNA or RNA by changes to the backbone of the molecule.In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides. The artificial nucleotides featured 2 fused aromatic rings.