Eur J - HAL
... and lysosomes and is related to the other aspartic proteases such a renin, pepsin and yeast protease A [1]. All these enzymes are synthesized as inactive precursors, which are then processed either auto-catalytically (e.g. pepsin) [1] or by other enzymes (e.g. renin) to remove an N-terminal pro-pept ...
... and lysosomes and is related to the other aspartic proteases such a renin, pepsin and yeast protease A [1]. All these enzymes are synthesized as inactive precursors, which are then processed either auto-catalytically (e.g. pepsin) [1] or by other enzymes (e.g. renin) to remove an N-terminal pro-pept ...
molecular cloning and characterization of snake venom
... cDNA phage library of SVMPs genes was constructed using mRNA from fresh venom glands of GPV. Each clone was recovered and sequenced. Full-length clones of GPV SVMPs were obtained using a 5-RACE method. Three novel metalloproteinases from GPV have been cloned and characterized. Detailed amino acid s ...
... cDNA phage library of SVMPs genes was constructed using mRNA from fresh venom glands of GPV. Each clone was recovered and sequenced. Full-length clones of GPV SVMPs were obtained using a 5-RACE method. Three novel metalloproteinases from GPV have been cloned and characterized. Detailed amino acid s ...
SCGN Blocking Peptide (C-term)
... Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml. Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Precautions This product is for research use ...
... Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml. Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Precautions This product is for research use ...
Molecular dynamics of serpins
... • It is a family of proteins characterised by a common molecular architecture • Most of the serpins are serine protease inhibitors, but some of them have other functions • Today, more than 500 serpins have been identified in animals, plants, bacteria and viruses ...
... • It is a family of proteins characterised by a common molecular architecture • Most of the serpins are serine protease inhibitors, but some of them have other functions • Today, more than 500 serpins have been identified in animals, plants, bacteria and viruses ...
Basic Peptide Bonds
... of cancers and similar diseases. In animal test subjects peptides have shown promise in detecting and targeting items such as LHRH and regulating the receptors. This process could be used to treat certain types of cancer, though the process for synthesizing these peptides or applying them in a pharm ...
... of cancers and similar diseases. In animal test subjects peptides have shown promise in detecting and targeting items such as LHRH and regulating the receptors. This process could be used to treat certain types of cancer, though the process for synthesizing these peptides or applying them in a pharm ...
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... All the samples from HIV-‐1 infected patients used in this work have been obtained from the HIV Unit at “Germans Trias i Pujol” University Hospital in Badalona. All the samples had their plasma ...
... All the samples from HIV-‐1 infected patients used in this work have been obtained from the HIV Unit at “Germans Trias i Pujol” University Hospital in Badalona. All the samples had their plasma ...
18_2014_1558_MOESM8_ESM
... confidence) was used as the threshold for protein identification. ‘Reverse database decoy analysis’ was used to estimate the protein identification false discovery rate. Using the specified search parameters, iTRAQ ratios and corresponding P-values were only reported for proteins identified by more ...
... confidence) was used as the threshold for protein identification. ‘Reverse database decoy analysis’ was used to estimate the protein identification false discovery rate. Using the specified search parameters, iTRAQ ratios and corresponding P-values were only reported for proteins identified by more ...
Overview of Microarray Types
... Figure 2. a) Demonstrates protein arrays which are based on microarray analysis of antigen-antibody interactions. Antigens are spotted onto glass slides. Antibodies which are tagged bind to antigens and emit fluorescent signal (shown as the yellow star) which can then be detected from the spot on th ...
... Figure 2. a) Demonstrates protein arrays which are based on microarray analysis of antigen-antibody interactions. Antigens are spotted onto glass slides. Antibodies which are tagged bind to antigens and emit fluorescent signal (shown as the yellow star) which can then be detected from the spot on th ...
In-gel Digestion
... Ltd.; version 2.2.1; in-house licensed). Searches were done with tryptic specificity, allowing two missed cleavages, or “non-specific cleavage” and a mass error tolerance of 5 ppm in MS spectra (i.e. FT-ICR data) and 0.5 Da for MS/MS ions (i.e. LTQ linear ion trap). Variable modifications included i ...
... Ltd.; version 2.2.1; in-house licensed). Searches were done with tryptic specificity, allowing two missed cleavages, or “non-specific cleavage” and a mass error tolerance of 5 ppm in MS spectra (i.e. FT-ICR data) and 0.5 Da for MS/MS ions (i.e. LTQ linear ion trap). Variable modifications included i ...
Master course KEMM03 Principles of Mass Spectrometric Protein
... Master course KEMM03 Principles of Mass Spectrometric Protein Characterization ...
... Master course KEMM03 Principles of Mass Spectrometric Protein Characterization ...
In Depth Analysis of Protein Amino Acid Sequence and PTMs with
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
Isofocusing Chromatography
... chromatography technique that separates protein according to differences in their isoelectric point (PI). •Its a polishing step for partially purified samples. •Its a high resolution technique and can be used for analytical separation. •We can be used this technique as a complementary technique when ...
... chromatography technique that separates protein according to differences in their isoelectric point (PI). •Its a polishing step for partially purified samples. •Its a high resolution technique and can be used for analytical separation. •We can be used this technique as a complementary technique when ...
Introduction of Microarray - genomics-lab
... finding and refining biological pathways Mutation and polymorphism detection ...
... finding and refining biological pathways Mutation and polymorphism detection ...
A photoactivatable green-fluorescent protein from the phylum
... siphonophore (Cnidaria, Hydrozoa) indicate that this mRNA was carried over from ingested material. This ctenophore species is known to prey on cnidarians, but we did not expect that mRNA would persist for as long as it did, and we regret our erroneous conclusion. What is remarkable about this situat ...
... siphonophore (Cnidaria, Hydrozoa) indicate that this mRNA was carried over from ingested material. This ctenophore species is known to prey on cnidarians, but we did not expect that mRNA would persist for as long as it did, and we regret our erroneous conclusion. What is remarkable about this situat ...
No Slide Title
... • Select extraction procedure according to source and location of protein • Use gentle procedures to minimize acidification and ...
... • Select extraction procedure according to source and location of protein • Use gentle procedures to minimize acidification and ...
ANIONIC EXCHANGE FRACTIONATION
... antibody. (B), On-chip immunoassay with the ubiquitin antibody: After binding the protein A and anti-ubiquitin antibody to the PS10 Chip urine samples from DM NP, DM WNP and healthy controls were loaded onto the chip and incubated for 1h under shaking. The matrix was added after washing. The binding ...
... antibody. (B), On-chip immunoassay with the ubiquitin antibody: After binding the protein A and anti-ubiquitin antibody to the PS10 Chip urine samples from DM NP, DM WNP and healthy controls were loaded onto the chip and incubated for 1h under shaking. The matrix was added after washing. The binding ...
Objective
... biological functions of proteins The study of the set of proteins produced by an organism with the aim of understanding their behaviour under varying conditions ...
... biological functions of proteins The study of the set of proteins produced by an organism with the aim of understanding their behaviour under varying conditions ...
Statistical Analysis Using Scaffold - Proteome Software
... descriptions of gene products in different databases ...
... descriptions of gene products in different databases ...
Functions and inhibitors of SecA, an essential protein in bacterial
... efficient, and like Prl suppressors, lack signal peptide specificity; they function in the absence of functional signal peptides. The addition of SecYEG-SecDF•YajC coverts the low affinity SecAonly channels to the high-affinity channels to restore efficiency and specificity. The 901 aminoacyl residu ...
... efficient, and like Prl suppressors, lack signal peptide specificity; they function in the absence of functional signal peptides. The addition of SecYEG-SecDF•YajC coverts the low affinity SecAonly channels to the high-affinity channels to restore efficiency and specificity. The 901 aminoacyl residu ...
Document
... • Select extraction procedure according to source and location of protein • Use gentle procedures to minimize acidification and ...
... • Select extraction procedure according to source and location of protein • Use gentle procedures to minimize acidification and ...
Fibrinolytic Bacterial Enzymes with Thrombolytic Activity
... secreted by humans include pepsin and protease for breakdown of proteins, lipase for fats, and amylase for carbohydrates. Cellulase, which helps with digestion of plant cells, is not produced by humans but is extracted from plant tissues as they are mechanically broken down. Plant-based foods are of ...
... secreted by humans include pepsin and protease for breakdown of proteins, lipase for fats, and amylase for carbohydrates. Cellulase, which helps with digestion of plant cells, is not produced by humans but is extracted from plant tissues as they are mechanically broken down. Plant-based foods are of ...